MNAzymes and gold nanoparticles as isothermal signal amplification strategy for visual detection of miRNA.

ABSTRACT: MicroRNAs (miRNAs) represent a class of small noncoding RNAs that are considered a novel emerging class of disease biomarkers in a variety of afflictions. Sensitive detection of miRNA is typically achieved using hybridization-based methods coupled with genetic amplification techniques. Although their sensitivity has improved, amplification techniques often present erroneous results due to their complexity. In addition, the use of these techniques is usually linked to the application of protein enzymes, the activity of which is dependent on the temperature and pH of the medium. To address these drawbacks, an alternative genetic enzyme for the highly sensitive detection of miRNAs is proposed in this work. Multicomponent nucleic acid enzymes (MNAzymes), coupled with the use of DNA-functionali

SUBMITTER: Sanchez-Visedo A 

PROVIDER: S-EPMC10352400 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature