Comparing derivatization reagents for quantitative LC-MS/MS analysis of a variety of vitamin D metabolites.

ABSTRACT: The present study systematically compares the sensitivity and selectivity of the analysis of multiple vitamin D metabolites after chemical derivatization using different reagents for liquid chromatography-tandem mass spectrometry (LC-MS/MS). Generally, chemical derivatization is applied to vitamin D metabolites to increase the ionization efficiency, which is particularly important for very low abundant metabolites. Derivatization can also improve the selectivity of the LC separation. A wide variety of derivatization reagents has been reported in recent years, but information on their relative performance and applicability to different vitamin D metabolites is, unfortunately, not available in the literature. To fill this gap, we investigated vitamin D₃, 3β-25-hydroxyvitamin D

SUBMITTER: Alexandridou A 

PROVIDER: S-EPMC10352403 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature