Staphylococcus epidermidis biofilms undergo metabolic and matrix remodeling under nitrosative stress.

ABSTRACT: Staphylococcus epidermidis is a commensal skin bacterium that forms host- and antibiotic-resistant biofilms that are a major cause of implant-associated infections. Most research has focused on studying the responses to host-imposed stresses on planktonic bacteria. In this work, we addressed the open question of how S. epidermidis thrives on toxic concentrations of nitric oxide (NO) produced by host innate immune cells during biofilm assembly. We analyzed alterations of gene expression, metabolism, and matrix structure of biofilms of two clinical isolates of S. epidermidis, namely, 1457 and RP62A, formed under NO stress conditions. In both strains, NO lowers the amount of biofilm mass and causes increased production of lactate and decreased acetate excretion from biofi

SUBMITTER: Oliveira AS 

PROVIDER: S-EPMC10352803 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature