Reproducibility of drug-induced effects on the contractility of an engineered heart tissue derived from human pluripotent stem cells.

ABSTRACT: Introduction: Engineered heart tissues (EHTs) are three-dimensional culture platforms with cardiomyocytes differentiated from human pluripotent stem cells (hPSCs) and were designed for assaying cardiac contractility. For drug development applications, EHTs must have a stable function and provide reproducible results. We investigated these properties with EHTs made with different tissue casting batches and lines of differentiated hPSC-cardiomyocytes and analyzed them at different times after being fabricated. Methods: A video-optical assay was used for measuring EHT contractile outputs, and these results were compared with results from motion traction analysis of beating hPSC-cardiomyocytes cultured as monolayers in two-dimensional cultures. The reproducibility of induced cont

SUBMITTER: Arefin A 

PROVIDER: S-EPMC10352809 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature