Guianensin, a Simulium guianense salivary protein, has broad anti-hemostatic and anti-inflammatory properties.

ABSTRACT:

Background

Salivary glands from blood-feeding arthropods secrete several molecules that inhibit mammalian hemostasis and facilitate blood feeding and pathogen transmission. The salivary functions from Simulium guianense, the main vector of Onchocerciasis in South America, remain largely understudied. Here, we have characterized a salivary protease inhibitor (Guianensin) from the blackfly Simulium guianense.

Materials and methods

A combination of bioinformatic and biophysical analyses, recombinant protein production, in vitro and in vivo experiments were utilized to characterize the molecula mechanism of action of Guianensin. Kinetics of Guianensin interaction with proteases involved in vertebrate inflammation and coagulation were carried out by surface plasmon

SUBMITTER: Valenzuela-Leon PC 

PROVIDER: S-EPMC10353047 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature