Study of the regulatory elements of the Ovalbumin gene promoter using CRISPR technology in chicken cells.

ABSTRACT:

Background

Hormone-dependent promoters are very efficient in transgene expression. Plasmid-based reporter assays have identified regulatory sequences of the Ovalbumin promoter that are involved in response to estrogen and have shown that the deletion of the steroid-dependent regulatory element (SDRE) and negative regulatory element (NRE) leads to a steroid-independent expression of a reporter. However, the functional roles of these regulatory elements within the native genomic context of the Ovalbumin promoter have not been evaluated.

Results

In this study, we show that the negative effects of the NRE element on the Ovalbumin gene can be counteracted by CRISPR interference. We also show that the CRISPR-mediated deletion of SDRE and NRE promoter elements in a non-oviduct cell

SUBMITTER: Yousefi Taemeh S 

PROVIDER: S-EPMC10353141 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature