Attenuation of PM_2.5-induced alveolar epithelial cells and lung injury through regulation of mitochondrial fission and fusion.

ABSTRACT:

Background

Exposure to particulate matter (PM) with an aerodynamic diameter less than 2.5 μm (PM_2.5) is a risk factor for developing pulmonary diseases and the worsening of ongoing disease. Mitochondrial fission and fusion are essential processes underlying mitochondrial homeostasis in health and disease. We examined the role of mitochondrial fission and fusion in PM_2.5-induced alveolar epithelial cell damage and lung injury. Key genes in these processes include dystrophin-related protein 1 (DRP1) and optic atrophy 1 (OPA1) respectively.

Methods

Alveolar epithelial (A549) cells were treated with PM_2.5 (32 µg/ml) in the presence and absence of Mdivi-1 (10µM, a DRP1 inhibitor) or BGP-15 (10µM, an OPA1 activator). Results were validated using DR

SUBMITTER: Liu Q 

PROVIDER: S-EPMC10353144 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature