High-Sensitivity Proteome-Scale Searches for Crosslinked Peptides Using CRIMP 2.0.

ABSTRACT: Crosslinking mass spectrometry (XL-MS) is a valuable technique for generating point-to-point distance measurements in protein space. However, cell-based XL-MS experiments require efficient software that can detect crosslinked peptides with sensitivity and controlled error rates. Many algorithms implement a filtering strategy designed to reduce the size of the database prior to mounting a search for crosslinks, but concern has been expressed over the possibility of reduced sensitivity using these strategies. We present a new scoring method that uses a rapid presearch method and a concept inspired by computer vision algorithms to resolve crosslinks from other conflicting reaction products. Searches of several curated crosslink datasets demonstrate high crosslink detection rates, and even the

SUBMITTER: Crowder DA 

PROVIDER: S-EPMC10353309 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature