Nutrient Limitation Sensitizes Pseudomonas aeruginosa to Vancomycin.

ABSTRACT: Traditional antibacterial screens rely on growing bacteria in nutrient-replete conditions which are not representative of the natural environment or sites of infection. Instead, screening in more physiologically relevant conditions may reveal novel activity for existing antibiotics. Here, we screened a panel of antibiotics reported to lack activity against the opportunistic Gram-negative bacterium, Pseudomonas aeruginosa, under low-nutrient and low-iron conditions, and discovered that the glycopeptide vancomycin inhibited the growth of P. aeruginosa at low micromolar concentrations through its canonical mechanism of action, disruption of peptidoglycan crosslinking. Spontaneous vancomycin-resistant mutants underwent activating mutations in the sensor kinase of the two-componen

SUBMITTER: Chan DCK 

PROVIDER: S-EPMC10353551 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature