Human Lacrimal Gland Derived Mesenchymal Stem Cells - Isolation, Propagation, and Characterization.

ABSTRACT:

Purpose

The existing treatment options for dry eye disease (DED) due to lacrimal gland (LG) dysfunction are mainly palliative. Mesenchymal stem cells (MSCs) based therapies and 3D-LG organoids have been explored as a curative option for LG regeneration in animal models. Human LG epithelial cultures are previously established and, here, we aim to isolate and characterize the spindle-shaped cells obtained from primary human LG cultures in order to unveil its MSC property.

Methods

Normal human lacrimal glands were obtained from individuals undergoing LG debulking surgery. The conditions for human LG-MSC culture were standardized to obtain pure population of LG-MSCs at passage 3. Population doubling time (PDT), expression of phenotypic markers, tri-lineage differentiation, colon

SUBMITTER: Jaffet J 

PROVIDER: S-EPMC10353750 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature