Potential risk of tamoxifen: gut microbiota and inflammation in mice with breast cancer.

ABSTRACT:

Objective

Tamoxifen is an effective anti-tumor medicine, but evidence has been provided on tamoxifen-related inflammation as well as its impact on gut microbiota. In this study, we aimed to investigate tamoxifen-induced gut microbiota and inflammation alteration.

Methods

We established a BC xenograft mouse model using the MCF-7 cell line. 16S rRNA gene sequencing was used to investigate gut microbiota. qRT-PCR, western blotting, and cytometric bead array were used to investigate inflammation-related biomarkers. Various bioinformatic approaches were used to analyze the data.

Results

Significant differences in gut microbial composition, characteristic taxa, and microbiome phenotype prediction were observed between control, model, and tamoxifen-treated mice. Furthermore,

SUBMITTER: Li H 

PROVIDER: S-EPMC10353877 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature