Gamma-aminobutyric acid (GABA) can affect physiological processes in preimplantation embryos via GABA_A and GABA_B receptors.

ABSTRACT:

Purpose

Several widely used substances (e.g., some therapeutics or food supplements) can act on gamma-aminobutyric acid (GABA) receptors, and we investigated whether the activation of these receptors could affect the preimplantation embryo.

Methods

Transcripts of all GABA receptor subunits and selected proteins were examined using quantitative RT-PCR and immunohistochemistry. To analyze the effects of receptor activation, in vitro culture of mouse preimplantation embryos with natural and synthetic GABA receptor ligands was used.

Results

We detected nine GABA receptor transcripts in mouse blastocysts and 14 GABA receptor transcripts in ovulated oocytes. The results of this study indicate that ionotropic GABA_A receptors can be formed from α5, β3, and γ3 (or δ

SUBMITTER: Kovarikova V 

PROVIDER: S-EPMC10354355 | biostudies-literature | 2023 Jan-Dec

REPOSITORIES: biostudies-literature