A multi-mineral intervention to counter pro-inflammatory activity and to improve the barrier in human colon organoids.

ABSTRACT: Introduction: Ulcerative colitis is a chronic inflammatory condition, and continuous inflammatory stimulus may lead to barrier dysfunction. The goal of this study was to assess barrier proteomic expression by a red algae-derived multi-mineral intervention in the absence or presence of pro-inflammatory insult. Methods: Human colon organoids were maintained in a control culture medium alone or exposed to lipopolysaccharide with a combination of three pro-inflammatory cytokines [tumor necrosis factor-α, interleukin-1β and interferon-γ (LPS-cytokines)] to mimic the environment in the inflamed colon. Untreated organoids and those exposed to LPS-cytokines were concomitantly treated for 14 days with a multi-mineral product (Aquamin^®) that has previously been shown to impr

SUBMITTER: Varani J 

PROVIDER: S-EPMC10354648 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature