Spectroelectrochemical behavior of parallel arrays of single vertically oriented Pseudomonas aeruginosa cells.

ABSTRACT: Pseudomonas aeruginosa is a Gram-negative opportunistic human pathogen responsible for a number of healthcare-associated infection. It is currently difficult to assess single cell behaviors of P. aeruginosa that might contribute to acquisition of antibiotic resistance, intercellular communication, biofilm development, or virulence, because mechanistic behavior is inferred from ensemble collections of cells, thus averaging effects over a population. Here, we develop and characterize a device that can capture and trap arrays of single P. aeruginosa cells in individual micropores in order to study their behaviors using spectroelectrochemistry. Focused ion beam milling is used to fabricate an array of micropores in a Au/dielectric/Au/SiO₂-containing multilayer s

SUBMITTER: Cutri AR 

PROVIDER: S-EPMC10355145 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature