Mechanisms of dihydromyricetin against hepatocellular carcinoma elucidated by network pharmacology combined with experimental validation.

ABSTRACT:

Context

Dihydromyricetin (DMY) is extracted from vine tea, a traditional Chinese herbal medicine with anti-cancer, liver protection, and cholesterol-lowering effects.

Objective

This study investigated the mechanism of DMY against hepatocellular carcinoma (HCC).

Materials and methods

Potential DMY, HCC, and cholesterol targets were collected from relevant databases. PPI networks were created by STRING. Then, the hub genes of co-targets, screened using CytoHubba. GO and KEGG pathway enrichment, were performed by Metascape. Based on the above results, a series of in vitro experiments were conducted by using 40-160 μM DMY for 24 h, including transwell migration/invasion assay, western blotting, and Bodipy stain assay.

Results

Network pharmacology identified

SUBMITTER: Zhang S 

PROVIDER: S-EPMC10355694 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature