FAVIS: Fast and versatile protocol for non-destructive metabarcoding of bulk insect samples.

ABSTRACT: Insects are diverse and sustain essential ecosystem functions, yet remain understudied. Recent reports about declines in insect abundance and diversity have highlighted a pressing need for comprehensive large-scale monitoring. Metabarcoding (high-throughput bulk sequencing of marker gene amplicons) offers a cost-effective and relatively fast method for characterizing insect community samples. However, the methodology applied varies greatly among studies, thus complicating the design of large-scale and repeatable monitoring schemes. Here we describe a non-destructive metabarcoding protocol that is optimized for high-throughput processing of Malaise trap samples and other bulk insect samples. The protocol details the process from obtaining bulk samples up to submitting libraries for sequenci

SUBMITTER: Iwaszkiewicz-Eggebrecht E 

PROVIDER: S-EPMC10356154 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature