Endothelin-3 is epigenetically silenced in endometrioid endometrial cancer.

ABSTRACT:

Purpose

Changes in the activity of endothelins and their receptors may promote neoplastic processes. They can be caused by epigenetic modifications and modulators, but little is known about endothelin-3 (EDN3), particularly in endometrial cancer. The aim of the study was to determine the expression profile of endothelin family and their interactions with miRNAs, and to assess the degree of EDN3 methylation.

Methods

The study enrolled 45 patients with endometrioid endometrial cancer and 30 patients without neoplastic changes. The expression profile of endothelins and their receptors was determined with mRNA microarrays and RT-qPCR. The miRNA prediction was based on the miRNA microarray experiment and the mirDB tool. The degree of EDN3 methylation was assessed by MSP.

Resul

SUBMITTER: Zmarzly N 

PROVIDER: S-EPMC10356642 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature