Small extracellular vesicles promote invadopodia activity in glioblastoma cells in a therapy-dependent manner.

ABSTRACT:

Purpose

The therapeutic efficacy of radiotherapy/temozolomide treatment for glioblastoma (GBM) is limited by the augmented invasiveness mediated by invadopodia activity of surviving GBM cells. As yet, however the underlying mechanisms remain poorly understood. Due to their ability to transport oncogenic material between cells, small extracellular vesicles (sEVs) have emerged as key mediators of tumour progression. We hypothesize that the sustained growth and invasion of cancer cells depends on bidirectional sEV-mediated cell-cell communication.

Methods

Invadopodia assays and zymography gels were used to examine the invadopodia activity capacity of GBM cells. Differential ultracentrifugation was utilized to isolate sEVs from conditioned medium and proteomic analyses were cond

SUBMITTER: Whitehead CA 

PROVIDER: S-EPMC10356899 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature