Triple combination of lomustine, temozolomide and irradiation reduces canine glioma cell survival in vitro.

ABSTRACT:

Background

Combined chemoradiation offers a promising therapeutic strategy for dogs with glioma. The alkylating agents temozolomide (TMZ) and lomustine (CCNU) penetrate the blood-brain barrier, and doses for dogs are established. Whether such combinations are clinically advantageous remains to be explored together with tumour-specific markers.

Objective

To investigate if triple combination of lomustine, temozolomide and irradiation reduces canine glioma cell survival in vitro.

Methods

We evaluated the sensitising effect of CCNU alone and in combination with TMZ-irradiation in canine glioma J3T-BG cells and long-term drug-exposed subclones by using clonogenic survival and proliferation assays. Bisulphite-SEQ and Western Blot were used to investigate molecular alteratio

SUBMITTER: Fuchs D 

PROVIDER: S-EPMC10357258 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature