Exposomic Biomonitoring of Polyphenols by Non-Targeted Analysis and Suspect Screening.

ABSTRACT: Polyphenols, prevalent in plants and fungi, are investigated intensively in nutritional and clinical settings because of their beneficial bioactive properties. Due to their complexity, analysis with untargeted approaches is favorable, which typically use high-resolution mass spectrometry (HRMS) rather than low-resolution mass spectrometry (LRMS). Here, the advantages of HRMS were evaluated by thoroughly testing untargeted techniques and available online resources. By applying data-dependent acquisition on real-life urine samples, 27 features were annotated with spectral libraries, 88 with in silico fragmentation, and 113 by MS¹ matching with PhytoHub, an online database containing >2000 polyphenols. Moreover, other exogenous and endogenous molecules were screened to measu

SUBMITTER: Oesterle I 

PROVIDER: S-EPMC10357401 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature