CanB, a Druggable Cellular Target in Mycobacterium tuberculosis.

ABSTRACT: Treatment against tuberculosis can lead to the selection of drug-resistant Mycobacterium tuberculosis strains. To tackle this serious threat, new targets from M. tuberculosis are needed to develop novel effective drugs. In this work, we aimed to provide a possible workflow to validate new targets and inhibitors by combining genetic, in silico, and enzymological approaches. CanB is one of the three M. tuberculosis β-carbonic anhydrases that catalyze the reversible reaction of CO₂ hydration to form HCO₃^- and H⁺. To this end, we precisely demonstrated that CanB is essential for the survival of the pathogen in vitro by constructing conditional mutants. In addition, to search for CanB inhibitors, conditional canB<

SUBMITTER: Degiacomi G 

PROVIDER: S-EPMC10357428 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature