Nanoparticle delivery of TFOs is a novel targeted therapy for HER2 amplified breast cancer.

ABSTRACT:

Purpose

The human EGFR2 (HER2) signaling pathway is one of the most actively studied targets in cancer transformation research. Ttriplex-forming oligonucleotides (TFOs) activate DNA damage and induce apoptosis. We aim to encapsulate TFO-HER2 with nano-particle ZW-128 to suppress breast cell growth in vitro and in vivo.

Experimental design

We designed a set of TFO fragments targeting HER2 and verified their effectiveness. We encapsulated TFO-HER2 in ZW-128 to form nano-drug TFO@ZW-128. Cell counting kit 8, flow cytometry, and western blotting were used to evaluate the effect of TFO@ZW-128 on cell proliferation and the expressions of related proteins. The ant-itumor effect of TFO@ZW-128 was evaluated in vivo using nude mice breast cancer model.

Results

TFO@ZW-128 had ef

SUBMITTER: Yang X 

PROVIDER: S-EPMC10357592 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature