Quantifying metabolic activity of Ascaris suum L3 using resazurin reduction.

ABSTRACT:

Background

Helminth infections are an important public health problem in humans and have an even greater impact on domestic animal and livestock welfare. Current readouts for anthelmintic drug screening assays are stage development, migration, or motility that can be subjective, laborious, and low in throughput. The aim of this study was to apply and optimize a fluorometric technique using resazurin for evaluating changes in the metabolic activity of Ascaris suum third-stage larvae (L3), a parasite of high economic relevance in swine.

Methods

Ascaris suum L3 were mechanically hatched from 6- to 8-week embryonated and sucrose-gradient-enriched eggs. Resazurin dye and A. suum L3 were titrated in 96-well microtiter plates, and resazurin reduction activity was assessed by fluoro

SUBMITTER: Kundik A 

PROVIDER: S-EPMC10357624 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature