Lessons from discovery of true ADAR RNA editing sites in a human cell line.

ABSTRACT:

Background

Conversion or editing of adenosine (A) into inosine (I) catalyzed by specialized cellular enzymes represents one of the most common post-transcriptional RNA modifications with emerging connection to disease. A-to-I conversions can happen at specific sites and lead to increase in proteome diversity and changes in RNA stability, splicing, and regulation. Such sites can be detected as adenine-to-guanine sequence changes by next-generation RNA sequencing which resulted in millions reported sites from multiple genome-wide surveys. Nonetheless, the lack of extensive independent validation in such endeavors, which is critical considering the relatively high error rate of next-generation sequencing, leads to lingering questions about the validity of the current compendiums of th

SUBMITTER: Wang F 

PROVIDER: S-EPMC10357658 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature