Ablation of cDC2 development by triple mutations within the Zeb2 enhancer.

ABSTRACT: The divergence of the common dendritic cell progenitor^1-3 (CDP) into the conventional type 1 and type 2 dendritic cell (cDC1 and cDC2, respectively) lineages^4,5 is poorly understood. Some transcription factors act in the commitment of already specified progenitors-such as BATF3, which stabilizes Irf8 autoactivation at the +32 kb Irf8 enhancer^4,6-but the mechanisms controlling the initial divergence of CDPs remain unknown. Here we report the transcriptional basis of CDP divergence and describe the first requirements for pre-cDC2 specification. Genetic epistasis analysis⁷ suggested that Nfil3 acts upstream of Id2, Batf3 and Zeb2 in cDC1 development but did not reveal its mechanism or targets. Analysis of newly generated NFIL3 reporter mice showed e

SUBMITTER: Liu TT 

PROVIDER: S-EPMC10358283 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature