Fidgetin interacting with microtubule end binding protein EB3 affects axonal regrowth in spinal cord injury.

ABSTRACT: Fidgetin, a microtubule-severing enzyme, regulates neurite outgrowth, axonal regeneration, and cell migration by trimming off the labile domain of microtubule polymers. Because maintenance of the microtubule labile domain is essential for axon initiation, elongation, and navigation, it is of interest to determine whether augmenting the microtubule labile domain via depletion of fidgetin serves as a therapeutic approach to promote axonal regrowth in spinal cord injury. In this study, we constructed rat models of spinal cord injury and sciatic nerve injury. Compared with spinal cord injury, we found that expression level of tyrosinated microtubules in the labile portion of microtubules continuously increased, whereas fidgetin decreased after peripheral nerve injury. Depletion of fidgetin enh

SUBMITTER: Ma C 

PROVIDER: S-EPMC10358656 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature