TLR7/TLR8 activation and susceptibility genes synergize to breach gut barrier in a mouse model of lupus.

ABSTRACT:

Background

Mounting evidence suggests that increased gut permeability, or leaky gut, and the resulting translocation of pathobionts or their metabolites contributes to the pathogenesis of Systemic Lupus Erythematosus. However, the mechanisms underlying the induction of gut leakage remain unclear. In this study, we examined the effect of a treatment with a TLR7/8 agonist in the B6.Sle1.Sle2.Sle3 triple congenic (TC) mouse, a spontaneous mouse model of lupus without gut leakage.

Materials and methods

Lupus-prone mice (TC), TC.Rag1^-/- mice that lack B and T cells, and congenic B6 healthy controls were treated with R848. Gut barrier integrity was assessed by measuring FITC-dextran in the serum following oral gavage. Claudin-1 and PECAM1 expression as wel

SUBMITTER: Ma L 

PROVIDER: S-EPMC10358848 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature