Dissimilar effects of stereoisomers and racemic hydroxychloroquine on Ca²⁺ oscillations in human induced pluripotent stem cell-derived cardiomyocytes.

ABSTRACT: All currently employed pharmaceutical formulations of hydroxychloroquine (HCQ) sulfate are a racemate, consisting of equal parts mixture of two stereoisomers: R(-)HCQ and S(+)HCQ sulfates. The aims of the current study were first, to obtain and characterize pure HCQ enantiomers. The separation and purification of free base HCQ enantiomers from the racemate form were performed using semi-preparative chiral high-performance liquid chromatography. Second, we compared the pharmacological properties of both optical isomers and racemic mixture on the intracellular Ca²⁺ oscillations employing an in vitro model of human cardiomyocytes derived from induced pluripotent stem cells (iPSCs). The results of the pharmacological investigations indicate that the racemic and pure stereoisomer for

SUBMITTER: Janicki PK 

PROVIDER: S-EPMC10359155 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature