Ontology highlight
ABSTRACT:
SUBMITTER: Valenzuela-Gomez F
PROVIDER: S-EPMC10359608 | biostudies-literature | 2023 Jul
REPOSITORIES: biostudies-literature
Valenzuela-Gómez Fernando F Arechaga Ignacio I Cabezón Elena E
Nucleic acids research 20230701 13
Bacterial conjugation is the main mechanism for the dissemination of antibiotic resistance genes. A single DNA strand of the conjugative plasmid is transferred across bacterial membranes covalently bound to a large multi-domain protein, named relaxase, which must be unfolded to traverse the secretion channel. Two tyrosine residues of the relaxase (Y18 and Y26 in relaxase TrwC) play an important role in the processing of conjugative DNA. We have used nanopore technology to uncover the unfolding s ...[more]