Thumb-domain dynamics modulate the functional repertoire of DNA-Polymerase IV (DinB).

ABSTRACT: In order to cope with the risk of stress-induced mutagenesis, cells in all kingdoms of life employ Y-family DNA polymerases to resolve resulting DNA lesions and thus maintaining the integrity of the genome. In Escherichia coli, the DNA polymerase IV, or DinB, plays this crucial role in coping with these type of mutations via the so-called translesion DNA synthesis. Despite the availability of several high-resolution crystal structures, important aspects of the functional repertoire of DinB remain elusive. In this study, we use advanced solution NMR spectroscopy methods in combination with biophysical characterization to elucidate the crucial role of the Thumb domain within DinB's functional cycle. We find that the inherent dynamics of this domain guide the recognition of double-stranded (d

SUBMITTER: Okeke DC 

PROVIDER: S-EPMC10359629 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature