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Fluorescence detection of three types of pollutants based on fluorescence resonance energy transfer and its comparison with colorimetric detection.


ABSTRACT: This study aimed at three representative pollutants, benzidine, cyromazine, and streptomycin, which were commonly used and posed a great threat to both environment and human health, mainly to explore a fast, simple, sensitive, visible naked-eye detection method. Colorimetric detection by gold nanoparticles (AuNPs) was first attempted. The cross-linking reaction occurred owing to the strong forces between the targets and AuNPs, leading to aggregation and color change. However, large-scale aggregation was easily formed and settled, which failed to achieve accurate quantification. Thus, AuNPs are considered to be used in fluorescence detection as reaction bridges. The introduction of AuNPs could effectively quench the fluorescence of Rhodamine B based on fluorescence resonance energy transfer

SUBMITTER: Kong Y 

PROVIDER: S-EPMC10359850 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature