Monitoring lineages of growing and dividing bacteria reveals an inducible memory of mar operon expression.

ABSTRACT: In Gram negative bacteria, the multiple antibiotic resistance or mar operon, is known to control the expression of multi-drug efflux genes that protect bacteria from a wide range of drugs. As many different chemical compounds can induce this operon, identifying the parameters that govern the dynamics of its induction is crucial to better characterize the processes of tolerance and resistance. Most experiments have assumed that the properties of the mar transcriptional network can be inferred from population measurements. However, measurements from an asynchronous population of cells can mask underlying phenotypic variations of single cells. We monitored the activity of the mar promoter in single Escherichia coli cells in linear micro-colonie

SUBMITTER: Guet CC 

PROVIDER: S-EPMC10359894 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature