RNautophagic regulation of DNMT3a-dependent DNA methylation by Linc00942 enhances chemoresistance in gastric cancer.

ABSTRACT:

Background

Energy balance has long been known to extend lifespans and inhibit carcinogenesis in multiple species by slowing age-related epigenetic changes while the underlying mechanisms remain largely unknown. Herein, we found that starvation activated autophagy to remodel the DNA methylation profile by inhibiting DNMT3a expression.

Methods

Illumina Infinium MethylationEPIC BeadChip and dot blot assay were performed to quantify the global DNA methylation level. Protein-RNA interactions were validated through RNA immunoprecipitation and RNA pull-down assay. In vitro and in vivo experiments were carried out to testify the effect of DNMT3a on chemoresistance.

Results

Autophagy is impaired in chemoresistance which was associated with differential DNA methylation and coul

SUBMITTER: Zhu L 

PROVIDER: S-EPMC10359971 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature