A Dual Workflow to Improve the Proteomic Coverage in Plasma Using Data-Independent Acquisition-MS.

ABSTRACT: Plasma is one of the most important and common matrices for clinical chemistry and proteomic analyses. Data-independent acquisition (DIA) mass spectrometry has enabled the simultaneous quantitative analysis of hundreds of proteins in plasma samples in support population and disease studies. Depletion of the highest abundant proteins is a common tool to increase plasma proteome coverage, but this strategy can result in the nonspecific depletion of protein subsets with which proteins targeted for depletion interact, adversely affecting their analysis. Our work using an antibody-based depletion column revealed significant complementarity not only in the identification of the proteins derived from depleted and undepleted plasma, but importantly also in the extent to which different proteins ca

SUBMITTER: Zhang S 

PROVIDER: S-EPMC10360210 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature