Oct1 cooperates with the Smad family of transcription factors to promote mesodermal lineage specification.

ABSTRACT: The transition between pluripotent and tissue-specific states is a key aspect of development. Understanding the pathways driving these transitions will facilitate the engineering of properly differentiated cells for experimental and therapeutic uses. Here, we showed that during mesoderm differentiation, the transcription factor Oct1 activated developmental lineage-appropriate genes that were silent in pluripotent cells. Using mouse embryonic stem cells (ESCs) with an inducible knockout of Oct1, we showed that Oct1 deficiency resulted in poor induction of mesoderm-specific genes, leading to impaired mesodermal and terminal muscle differentiation. Oct1-deficient cells exhibited poor temporal coordination of the induction of lineage-specific genes and showed inappropriate developmental lineag

SUBMITTER: Perovanovic J 

PROVIDER: S-EPMC10360295 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature