Growth Feedback Confers Cooperativity in Resource-Competing Synthetic Gene Circuits.

ABSTRACT: Modularity is a key concept in designing synthetic gene circuits, as it allows for constructing complex molecular systems using well-characterized building blocks. One of the major challenges in this field is that these modular components often do not function as expected when assembled into larger circuits. One of the major issues is caused by resource competition, where multiple genes in the circuit compete for the same limited cellular resources, such as transcription factors and ribosomes. In addition, the mutual inhibition between synthetic gene circuits and cell growth results in growth feedback that significantly impacts its host-circuit dynamics. However, the complexity of the gene circuit dynamics under intertwined resource competition and growth feedback is not fully understood.

SUBMITTER: Melendez-Alvarez JR 

PROVIDER: S-EPMC10361397 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature