Wide-field fluorescence lifetime imaging of neuron spiking and subthreshold activity in vivo.

ABSTRACT: The development of voltage-sensitive fluorescent probes suggests fluorescence lifetime as a promising readout for electrical activity in biological systems. Existing approaches fail to achieve the speed and sensitivity required for voltage imaging in neuroscience applications. We demonstrated that wide-field electro-optic fluorescence lifetime imaging microscopy (EO-FLIM) allows lifetime imaging at kilohertz frame-acquisition rates, spatially resolving action potential propagation and subthreshold neural activity in live adult Drosophila. Lifetime resolutions of <5 picoseconds at 1 kilohertz were achieved for single-cell voltage recordings. Lifetime readout is limited by photon shot noise, and the method provides strong rejection of motion artifacts and technical noise sources. Reco

SUBMITTER: Bowman AJ 

PROVIDER: S-EPMC10361454 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature