Scalable biomimetic sensing system with membrane receptor dual-monolayer probe and graphene transistor arrays.

ABSTRACT: Affinity-based biosensing can enable point-of-care diagnostics and continuous health monitoring, which commonly follows bottom-up approaches and is inherently constrained by bioprobes' intrinsic properties, batch-to-batch consistency, and stability in biofluids. We present a biomimetic top-down platform to circumvent such difficulties by combining a "dual-monolayer" biorecognition construct with graphene-based field-effect-transistor arrays. The construct adopts redesigned water-soluble membrane receptors as specific sensing units, positioned by two-dimensional crystalline S-layer proteins as dense antifouling linkers guiding their orientations. Hundreds of transistors provide statistical significance from transduced signals. System feasibility was demonstrated with rSbpA-ZZ/CXCR4^QTY<

SUBMITTER: Qing R 

PROVIDER: S-EPMC10361598 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature