Engineered exosomes reprogram Gli1⁺ cells in vivo to prevent calcification of vascular grafts and autologous pathological vessels.

ABSTRACT: Calcification of autologous pathological vessels and tissue engineering blood vessels (TEBVs) is a thorny problem in clinic. However, there is no effective and noninvasive treatment that is available against the calcification of TEBVs and autologous pathological vessels. Gli1⁺ cells are progenitors of smooth muscle cells (SMCs) and can differentiate into osteoblast-like cells, leading to vascular calcification. Our results showed that the spatiotemporal distribution of Gli1⁺ cells in TEBVs was positively correlated with the degree of TEBV calcification. An anticalcification approach was designed consisting of exosomes derived from mesenchymal stem cells delivering lncRNA-ANCR to construct the engineered exosome-Ancr/E7-EXO. The results showed that Ancr/E7-EXO e

SUBMITTER: Yan J 

PROVIDER: S-EPMC10361604 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature