Fluid extraction from the left-right organizer uncovers mechanical properties needed for symmetry breaking.

ABSTRACT: Humans and other vertebrates define body axis left-right asymmetry in the early stages of embryo development. The mechanism behind left-right establishment is not fully understood. Symmetry breaking occurs in a dedicated organ called the left-right organizer (LRO) and involves motile cilia generating fluid-flow therein. However, it has been a matter of debate whether the process of symmetry breaking relies on a chemosensory or a mechanosensory mechanism (Shinohara et al., 2012). Novel tailored manipulations for LRO fluid extraction in living zebrafish embryos allowed us to pinpoint a physiological developmental period for breaking left-right symmetry during development. The shortest critical time-window was narrowed to one hour and characterized by a mild counterclockwise flow. The experim

SUBMITTER: Sampaio P 

PROVIDER: S-EPMC10361723 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature