SHARPER-DOSY: Sensitivity enhanced diffusion-ordered NMR spectroscopy.

ABSTRACT: Since its discovery in mid-20^th century, the sensitivity of Nuclear Magnetic Resonance (NMR) has increased steadily, in part due to the design of new, sophisticated NMR experiments. Here we report on a liquid-state NMR methodology that significantly increases the sensitivity of diffusion coefficient measurements of pure compounds, allowing to estimate their sizes using a much reduced amount of material. In this method, the diffusion coefficients are being measured by analysing narrow and intense singlets, which are invariant to magnetic field inhomogeneities. The singlets are obtained through signal acquisition embedded in short (<0.5 ms) spin-echo intervals separated by non-selective 180° or 90° pulses, suppressing the chemical shift evolution of resonances and their splitting

SUBMITTER: Peat G 

PROVIDER: S-EPMC10361965 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature