Description of lipase producing novel yeast species Debaryomyces apis f.a., sp. nov. and a modified pH indicator dye-based method for the screening of lipase producing microorganisms.

ABSTRACT: Four yeast strains were isolated from the gut of stingless bee, collected in Churdhar, Himachal Pradesh, India. Physiological characterization, morphological examination, and sequence analysis of small subunit ribosomal RNA (18S rRNA) genes, internal transcribed spacer (ITS) region, and D1/D2 domain of the large subunit rRNA gene revealed that the four strains isolated from the gut of stingless bee belonged to the Debaryomyces clade. Strain CIG-23H^T showed sequence divergence of 7.5% from Debaryomyces nepalensis JCM 2095^T, 7.8% from Debaryomyces udenii JCM 7855^T, and Debaryomyces coudertii JCM 2387^T in the D1/D2 domain. In the ITS region sequences, strain CIG-23H^T showed a 15% sequence divergence from Debaryomyces nepalensis JCM 2095

SUBMITTER: Kumari A 

PROVIDER: S-EPMC10362016 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature