Metformin restores cognitive dysfunction and histopathological deficits in an animal model of sporadic Alzheimer's disease.

ABSTRACT:

Background

Metformin has been introduced as a neuroprotective agent in recent years. Here we evaluate the therapeutic effects of metformin in sporadic mouse model of Alzheimer's disease (SAD).

Methods

AD was induced by streptozotocin (STZ, 0.5 mg/kg) on days 1 and 3. Metformin (MET, 200 mg/kg per day) was used for two weeks. Novel objective recognition (NOR) and Barnes Maze test were used to test the learning and memory. Nissl staining was used as s histological method for counting the dying neurons in different regions of hippocampus. Immunofluorescence staining against glial fibrillary acidic protein (GFAP), ionized calcium binding adaptor molecule 1 (Iba1) and NeuN were used to visualize reactive astrocytes, microglia and neurons, respectively.

Results

In NOR test,

SUBMITTER: Rabieipoor S 

PROVIDER: S-EPMC10362193 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature