Hypoxia-based classification and prognostic signature for clinical management of hepatocellular carcinoma.

ABSTRACT:

Objective

Intratumoral hypoxia is an essential feature of hepatocellular carcinoma (HCC). Herein, we investigated the hypoxia-based heterogeneity and relevant clinical implication in HCC.

Methods

Three HCC cohorts: TCGA-LIHC, LICA-FR, and LIRI-JP were retrospectively gathered. Consensus clustering analysis was utilized for hypoxia-based classification based upon transcriptome of hypoxia genes. Through LASSO algorithm, a hypoxia-relevant prognostic signature was built. Immunotherapeutic response was inferred through analyzing immune checkpoints, T cell inflamed score, TIDE score, and TMB score. RNF145 expression was measured in normoxic or hypoxic HCC cells. In RNF145-knockout cells, CCK-8, TUNEL, and scratch tests were implemented.

Results

HCC patients were classified

SUBMITTER: Li K 

PROVIDER: S-EPMC10362578 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature