Mechanical force induces mitophagy-mediated anaerobic oxidation in periodontal ligament stem cells.

ABSTRACT:

Background

The preference for glucose oxidative mode has crucial impacts on various physiological activities, including determining stem cell fate. External mechanical factors can play a decisive role in regulating critical metabolic enzymes and pathways of stem cells. Periodontal ligament stem cells (PDLSCs) are momentous effector cells that transform mechanical force into biological signals during the reconstruction of alveolar bone. However, mechanical stimuli-induced alteration of oxidative characteristics in PDLSCs and the underlying mechanisms have not been fully elucidated.

Methods

Herein, we examined the expression of LDH and COX4 by qRT-PCR, western blot, immunohistochemistry and immunofluorescence. We detected metabolites of lactic acid and reactive oxygen species

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC10362665 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature