DNA barcoding of black flies (Diptera: Simuliidae) in Indonesia.

ABSTRACT:

Background

DNA barcoding is a valuable taxonomic tool for rapid and accurate species identification and cryptic species discovery in black flies. Indonesia has 143 nominal species of black flies, but information on their biological aspects, including vectorial capacity and biting habits, remains underreported, in part because of identification problems. The current study represents the first comprehensive DNA barcoding of Indonesian black flies using mitochondrial cytochrome c oxidase subunit I (COI) gene sequences.

Methods

Genomic DNA of Indonesian black fly samples were extracted and sequenced, producing 86 COI sequences in total. Two hundred four COI sequences, including 118 GenBank sequences, were analysed. Maximum likelihood (ML) and Bayesian inference (BI) trees were c

SUBMITTER: Hew YX 

PROVIDER: S-EPMC10362752 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature