Melanoma cells induce dedifferentiation and metabolic changes in adipocytes present in the tumor niche.

ABSTRACT:

Background

One of the factors that affect the progression of melanoma is the tumor microenvironment, which consists of cellular elements, extracellular matrix, acidification, and a hypoxic state. Adipocytes are one of the types of cell present in the niche and are localized in the deepest layer of the skin. However, the relationship between fat cells and melanoma remains unclear.

Methods

We assessed the influence of melanoma cells on adipocytes using an indirect coculture system. We estimated the level of cancer-associated adipocyte (CAA) markers through quantitative PCR analysis. The fibroblastic phenotype of CAAs was confirmed by cell staining and western blotting analysis. The lipid content was estimated by lipid detection in CAAs using LipidSpot and by quantitative analy

SUBMITTER: Simiczyjew A 

PROVIDER: S-EPMC10363323 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature