Vascular protection afforded by zinc supplementation in human coronary artery smooth muscle cells mediated by NRF2 signaling under hypoxia/reoxygenation.

ABSTRACT: Zinc (Zn) has antioxidant, anti-inflammatory and anti-proliferative actions, with Zn dysregulation associated with coronary ischemia/reperfusion injury and smooth muscle cell dysfunction. As the majority of studies concerning Zn have been conducted under non-physiological hyperoxic conditions, we compare the effects of Zn chelation or supplementation on total intracellular Zn content, antioxidant NRF2 targeted gene transcription and hypoxia/reoxygenation-induced reactive oxygen species generation in human coronary artery smooth muscle cells (HCASMC) pre-adapted to hyperoxia (18 kPa O₂) or normoxia (5 kPa O₂). Expression of the smooth muscle marker SM22-α was unaffected by lowering pericellular O₂, whereas calponin-1 was significantly upregulated in cells un

SUBMITTER: Yang F 

PROVIDER: S-EPMC10363453 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature