Rapid identification of bacteria using a multiplex polymerase chain reaction system for acute abdominal infections.

ABSTRACT:

Purpose

Acute abdominal infections can be fatal if the causative organism (s) are misidentified. The spread of antimicrobial-resistant bacteria has become a serious problem worldwide, making antibiotic selection extremely difficult. Using quantitative metagenomic analysis, we evaluated a commercial multiplex polymerase chain reaction (PCR) system (FilmArray™, bioMérieux, Marcy-l'Étoile, France) for the rapid identification of causative bacteria.

Methods

The cases of 10 patients with acute abdominal infections were enrolled in this retrospective study. There were six cases of perforated peritonitis and four cases of intraabdominal abscess. Fluid collected from the acute surgical abdominal infections were examined.

Results

All specimens tested positive for microorganism

SUBMITTER: Kakizaki N 

PROVIDER: S-EPMC10363666 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature