Quantitative multiple fragment monitoring with enhanced in-source fragmentation/annotation mass spectrometry.

ABSTRACT: Analytical techniques with high sensitivity and selectivity are essential to the quantitative analysis of clinical samples. Liquid chromatography coupled to tandem mass spectrometry is the gold standard in clinical chemistry. However, tandem mass spectrometers come at high capital expenditure and maintenance costs. We recently showed that it is possible to generate very similar results using a much simpler single mass spectrometry detector by performing enhanced in-source fragmentation/annotation (EISA) combined with correlated ion monitoring. Here we provide a step-by-step protocol for optimizing the analytical conditions for EISA, so anyone properly trained in liquid chromatography-mass spectrometry can follow and apply this technique for any given analyte. We exemplify the approach by u

SUBMITTER: Bernardo-Bermejo S 

PROVIDER: S-EPMC10364092 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature